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Simply put your card with your existing installation in to a USB card reader and get the correct drive letter from Windows Explorer. Make sure the correct drive letter is selected in WinDisk32. Give the image a name like RetroPie_Backup_4-2-2015.img. Click read.

After waiting what can seem like an eternity, you will have your backup image.

To put your backup image on a card, the process is the same as writing the initial RetroPie image. Put your clean card in the card reader. Make sure you select the correct drive letter. Select your RetroPie_Backup_4-2-2015.img. Click write.

Wait another eternity and you should have a copy of your original installation.